apc conjugated nti cd86 (Miltenyi Biotec)
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Miltenyi Biotec
apc conjugated nti cd86
Apc Conjugated Nti Cd86, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86+apc/CD86+Antibody%2C+anti-mouse%2C+REAfinity/10__1002_slash_advs__202522278-298-5-8
Average 94 stars, based on 19 article reviews
Apc Conjugated Nti Cd86, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd86+apc/CD86+Antibody%2C+anti-mouse%2C+REAfinity/10__1002_slash_advs__202522278-298-5-8
Average 94 stars, based on 19 article reviews
apc conjugated nti cd86 - by Bioz Stars,
2026-09
94/100 stars
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Staining:Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice Article Snippet: Cells were then resuspended at a cellular density of 10 6 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130-110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110-505), Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice. Article Snippet: Cells were then resuspended at a cellular density of 106 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130- 110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110- 505), Article Title: Active LXR signaling, coupled with elevated mitochondrial and glycolytic metabolism contributes to GM-CSF–induced trained immunity Article Snippet: .. The cells were washed with PBS and stained with surface markers for CD80-FITC (Biolegend, #305206), Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization. Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 105 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 ◦C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), CD54APC (Miltenyi Biotec, #130-121-342), and Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 10 5 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 °C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), CD54-APC (Miltenyi Biotec, #130-121-342), and Article Title: Modelling of the multicellular tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) on a fit-for-purpose biochip for preclinical drug discovery. Article Snippet: .. After spheroid digestion with Accutase (#A1110501, Gibco), the cells were stained with the following antibodies according to manufactures instructions; CD163-PE (#130-112-286, Miltenyi Biotec), CD11b-VioBlue (#130-110-616, Miltenyi Biotec), FACS:Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice Article Snippet: Cells were then resuspended at a cellular density of 10 6 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130-110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110-505), Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice. Article Snippet: Cells were then resuspended at a cellular density of 106 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130- 110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110- 505), Article Title: NF-κB and TET2 promote macrophage reprogramming in hypoxia that overrides the immunosuppressive effects of the tumor microenvironment Article Snippet: .. Then, 10 5 cells per condition were incubated in FACS buffer for 20 min at 4°C in the dark with optimal concentration of the following fluorophore-conjugated antibodies: HLA-DR-PE/Cy7 (BioLegend, clone L246), Activation Assay:Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice Article Snippet: Cells were then resuspended at a cellular density of 10 6 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130-110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110-505), Article Title: Immunogenicity of PE18, PE31, and PPE26 proteins from Mycobacterium tuberculosis in humans and mice. Article Snippet: Cells were then resuspended at a cellular density of 106 cells/mL and plated into a 24-well tissue culture microplate (1mL per well) in CellGenix DC medium (CellGenix, Cat.N° 20801-0500) added with Gentamycin, IL-4 (Miltenyi, 130-093-866) and GM-CSF (Miltenyi, 130-093-922) for 5 days. .. At day 5, cells were stained for FACS analysis with several DC activation markers to assess their immature dendritic cell (iDC) state: CD14-FITC (Miltenyi, 130- 110-518), CD40-BV510 (BD Biosciences, 563456), CD80-BV421 (BD Biosciences, 564160), CD83-PE-Vio 770 (Miltenyi, 130-110- 505), Incubation:Article Title: NF-κB and TET2 promote macrophage reprogramming in hypoxia that overrides the immunosuppressive effects of the tumor microenvironment Article Snippet: .. Then, 10 5 cells per condition were incubated in FACS buffer for 20 min at 4°C in the dark with optimal concentration of the following fluorophore-conjugated antibodies: HLA-DR-PE/Cy7 (BioLegend, clone L246), Article Title: Incorporating immune cell surrogates into a full-thickness tissue equivalent of human skin to characterize dendritic cell activation. Article Snippet: Surface marker detection via flow cytometry Cells were harvested after differentiation or treatment and washed in autoMacs Running Buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany). .. At least 1 × 105 cells for each antibody panel were transferred to 96-well u-bottom plates and incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130- 113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-948), CD1a-PE (Miltenyi Biotec, #130-112-022); CD207-PE-Vio770 (Miltenyi Biotec, #130-112-370), CD54-APC (Miltenyi Biotec, #130-121-342); Concentration Assay:Article Title: NF-κB and TET2 promote macrophage reprogramming in hypoxia that overrides the immunosuppressive effects of the tumor microenvironment Article Snippet: .. Then, 10 5 cells per condition were incubated in FACS buffer for 20 min at 4°C in the dark with optimal concentration of the following fluorophore-conjugated antibodies: HLA-DR-PE/Cy7 (BioLegend, clone L246), Control:Article Title: Incorporating immune cell surrogates into a full-thickness tissue equivalent of human skin to characterize dendritic cell activation. Article Snippet: Surface marker detection via flow cytometry Cells were harvested after differentiation or treatment and washed in autoMacs Running Buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany). .. At least 1 × 105 cells for each antibody panel were transferred to 96-well u-bottom plates and incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130- 113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-948), CD1a-PE (Miltenyi Biotec, #130-112-022); CD207-PE-Vio770 (Miltenyi Biotec, #130-112-370), CD54-APC (Miltenyi Biotec, #130-121-342); Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization. Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 105 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 ◦C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), CD54APC (Miltenyi Biotec, #130-121-342), and Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 10 5 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 °C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), CD54-APC (Miltenyi Biotec, #130-121-342), and |
